Visium Spatial Platform by 10x Genomics
The Visium Spatial Platform is an advanced spatial transcriptomics technology enabling whole transcriptome spatial gene expression analysis at single cell-scale resolution with high spatial fidelity. Compatible with multiple tissue types including fresh frozen, fixed frozen, and FFPE, this platform provides deep insights into tissue biology through histology-friendly workflows and powerful analysis software.
Buy Now📝 General Description
Visium delivers unbiased, whole transcriptome spatial gene expression profiling allowing researchers to study gene expression in the tissue context. It offers flexible assay options such as Visium Standard and Visium HD with different spatial resolutions (55 micron spots for Standard and 2 micron barcoded squares for HD). The platform includes specialized slides, reagents, and instrument (Visium CytAssist) for high-quality spatial analysis.
⚙️ How Does It Work
Tissue samples (fresh frozen, fixed frozen, or FFPE) are sectioned and placed on Visium slides. The slides feature barcoded spots that capture released RNA transcripts after probe hybridization or poly(A) capture depending on the assay type. The captured transcripts undergo library preparation and next-generation sequencing. Data from sequencing is spatially mapped back to their original locations on the tissue allowing visualization of gene expression with software tools such as Space Ranger and Loupe Browser.
🌟 Benefits
High Spatial Resolution
Down to single-cell scale with Visium HD for unmatched detail.
Multiple Tissue Compatibility
Works with fresh frozen, fixed frozen, and FFPE samples for versatility.
User-Friendly Software
Space Ranger and Loupe Browser empower intuitive spatial gene analysis.
Histology-Friendly
Compatible with standard H&E & IF staining to link morphology with expression.
Flexible Assays
Options range from wide coverage (Standard) to detailed single-cell resolution (HD & FFPE v2).
Powerful Insights
Facilitates tumor microenvironment, drug response, and developmental biology studies.
💾 Software and Data Analysis
The platform includes Space Ranger for alignment, gene expression quantification, and clustering, plus Loupe Browser for interactive spatial data visualization. These user-friendly tools allow exploration of gene signatures, spatial variability, cell type identification, and tissue morphology integration. They enable quick data processing and figure generation optimized for scientific publications.
🧬 Assay Options
- Visium Standard: Whole transcriptome analysis with 55 μm spots, species agnostic, optimized for fresh frozen samples.
- Visium HD: Achieves single-cell scale resolution with 2 micron barcoded features, probe-based chemistry, and compatibility with fresh frozen, fixed frozen, and FFPE.
- Visium FFPE v2 with CytAssist: Sequencing-based spatial profiling using probe hybridization for human and mouse FFPE or frozen tissue sections.
🔬 Sample Compatibility and Preparation
Supports fresh frozen, fixed frozen, and FFPE tissue sections. Compatible with standard histological stains such as hematoxylin and eosin (H&E) and immunofluorescence (IF) for protein co-detection. Sample preparation workflows are compatible with processing in standard histology labs, making integration seamless for established procedures.
📏 Data Quality and Resolution
Provides high spatial fidelity with histology-friendly workflows. Standard Visium has 55 μm resolution spots, while Visium HD achieves single-cell scale resolution with 2x2 μm features without gaps. Visium HD 3' further enhances discovery capabilities including isoform, TCR/BCR, and SNV detection.
🔍 Applications
Used for cutting-edge research and discovery in:
- Cancer research: study tumor microenvironment heterogeneity.
- Drug discovery: investigate drug response within the tissue context.
- Developmental biology: analyze gene expression patterning during development.
- Immune cell characterization and spatial multi-omics studies.
🏭 Manufacturing and Origin
Developed and manufactured by 10x Genomics, USA, the Visium Spatial Platform is backed by extensive support infrastructure including user training, technical documentation, and customer support to ensure smooth adoption and successful research outcomes.
👍 Pros
- High spatial resolution down to single-cell scale with Visium HD
- Supports multiple tissue types including FFPE, fresh frozen, and fixed frozen
- Whole transcriptome coverage across broad range of species and tissues
- Histology-friendly workflow compatible with standard H&E and IF staining
- User-friendly analysis software (Space Ranger and Loupe Browser) requiring minimal bioinformatics skill
- Enables integration of spatial gene expression with histological morphology
- Flexible assay portfolio for comprehensive spatial discovery and targeted analysis
- Compatible with established sequencing platforms (Illumina NovaSeq X Plus and Element AVITI)
- Strong support resources including tutorials, data sets, publications, and grant writing assistance
⭐ User Reviews
Katie Olson
I Didn't Think This Would Work.. But It Did!
I didn't want to try another supplement because none of the ones on Amazon worked for me. But after seeing the evidence, I thought ‘what the heck.’ And what do you know? All the floaters, black spots, and blurriness has cleared up. I can drive at night again, knit my granddaughter a sweater, and my optometrist was shocked to see I’m now reading TWO lines better on the eye chart.
Sandra Garcia
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